Overview

  • Product name
    Anti-Paxillin (phospho Y31) antibody
    See all Paxillin primary antibodies
  • Description
    Rabbit polyclonal to Paxillin (phospho Y31)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 31.

  • Positive control
    • NM_MG cells transfected with either the wild-type EGFP-tagged apxillin or a Y315F mutant.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Please see Grace et al., 2003.

WB 1/1000. Predicted molecular weight: 68 kDa.

Target

  • Function
    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • Sequence similarities
    Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

Images

  • Extracts prepared from NMµMG cells transfected with either the wild-type EGFP-tagged paxillin or a site directed mutant, in which tyrosine 31 was changed to phenylalanine, were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with 1% BSA, followed by incubation with 0.5 µg/mL anti-paxillin [pY31] antibody. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence using the Tropix WesternStar detection method and Kodak BioMax ultraclear film. The data show detection of paxillin phosphorylation with the wild-type but not the Y31F mutant recombinant proteins. Note that the recombinant EGFP-tagged paxillin proteins run at a higher Mr as opposed to the endogenous protein and that in both cell types that the antibody recognizes the endogenous wild-type paxillin protein, which migrates at the expected Mr of 68 kDa. Ext
  • Colocalization of phospho Paxillin and actin in TGF-beta treated NMµMG cells. Green = actin stress fibers, Orange = Paxillin [pY31], Yellow = colocalization.

References

This product has been referenced in:
  • Gill MB  et al. KSHV-TK is a tyrosine kinase that disrupts focal adhesions and induces Rho-mediated cell contraction. EMBO J 34:448-65 (2015). Read more (PubMed: 25471072) »
  • Kawada I  et al. Paxillin mutations affect focal adhesions and lead to altered mitochondrial dynamics: relevance to lung cancer. Cancer Biol Ther 14:679-91 (2013). IHC . Read more (PubMed: 23792636) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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