• Product name

    Anti-Paxillin (phospho Y31) antibody
    See all Paxillin primary antibodies
  • Description

    Rabbit polyclonal to Paxillin (phospho Y31)
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 31.

  • Positive control

    • ICC/IF NIH/3T3 treated with PDGF or TGF-beta. WB: NM_MG cells transfected with the wild-type EGFP-tagged paxillin, A431 cell lysate treated with pervanadate, HeLa cell lysate treated with TNF alpha, NIH/3T3 cell lysate treated with PDGF, and A549 cell lysate treated with PMA.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab4832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Please see Grace et al., 2003.

ICC/IF 1/250.
WB 1/1000. Predicted molecular weight: 68 kDa.


  • Function

    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • Sequence similarities

    Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • Post-translational

    Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all


  • Immunocytochemistry/ Immunofluorescence of NIH/3T3 (mouse embryo fibroblast cell line) treated with 50 ng of PDGF for 10 minutes with ab4832 (1/250). A Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate (1/2000) was used as a secondary (Green). DAPI (Blue) was used as a counter stain. F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel e is untreaded cells and panel f had no primary antibody. 

  • All lanes : Anti-Paxillin (phospho Y31) antibody (ab4832)

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate treated for 1 Hr with 100 µM of Pervanadate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated for 20 minutes with 50 ng/mL of TNF alpha
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate treated for 10 minutes with 50 ng/mL of PDGF
    Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 8 : A549 (human lung carcinoma cell line) whole cell lysate treated for 20 minutes with 200 nM of PMA

    Lysates/proteins at 20 µl per lane.

    All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

    ab4832 was diluted between 1/500 and 1/2000. 

  • Extracts prepared from NMµMG cells transfected with either the wild-type EGFP-tagged paxillin or a site directed mutant, in which tyrosine 31 was changed to phenylalanine, were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with 1% BSA, followed by incubation with 0.5 µg/mL anti-paxillin [pY31] antibody. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence using the Tropix WesternStar detection method and Kodak BioMax ultraclear film. The data show detection of paxillin phosphorylation with the wild-type but not the Y31F mutant recombinant proteins. Note that the recombinant EGFP-tagged paxillin proteins run at a higher Mr as opposed to the endogenous protein and that in both cell types that the antibody recognizes the endogenous wild-type paxillin protein, which migrates at the expected Mr of 68 kDa. Ext
  • Colocalization of phospho Paxillin and actin in TGF-beta treated NMµMG cells. Green = actin stress fibers, Orange = Paxillin [pY31], Yellow = colocalization.


This product has been referenced in:

  • Gill MB  et al. KSHV-TK is a tyrosine kinase that disrupts focal adhesions and induces Rho-mediated cell contraction. EMBO J 34:448-65 (2015). Read more (PubMed: 25471072) »
  • Kawada I  et al. Paxillin mutations affect focal adhesions and lead to altered mitochondrial dynamics: relevance to lung cancer. Cancer Biol Ther 14:679-91 (2013). IHC . Read more (PubMed: 23792636) »
See all 4 Publications for this product

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