Overview

  • Product name

    Anti-Paxillin (phospho Y31) antibody - N-terminal
    See all Paxillin primary antibodies
  • Description

    Rabbit polyclonal to Paxillin (phospho Y31) - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Paxillin (N terminal) (phospho Y31). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: P49023

  • Positive control

    • WB: HeLa whole cell extracts; Pervanadate treated A431 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • Sequence similarities

    Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

Images

  • All lanes : Anti-Paxillin (phospho Y31) antibody - N-terminal (ab229644) at 1/2000 dilution

    Lane 1 : Non-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 2 : Paxillin shRNA-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 65 kDa



    7.5% SDS-PAGE

  • All lanes : Anti-Paxillin (phospho Y31) antibody - N-terminal (ab229644) at 1/1000 dilution

    Lane 1 : Untreated A431 (Human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : A431 (Human epidermoid carcinoma cell line) treated with 100 µm pervanadate for 30 minutes, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 65 kDa



    7.5% SDS-PAGE

References

ab229644 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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