Anti-Paxillin (phospho Y88) antibody (ab131446)
Key features and details
- Rabbit polyclonal to Paxillin (phospho Y88)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Paxillin (phospho Y88) antibody
See all Paxillin primary antibodies -
Description
Rabbit polyclonal to Paxillin (phospho Y88) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Paxillin (phospho Y88) conjugated to keyhole limpet haemocyanin.
Database link: NP_001074324.1 -
Positive control
- HT29 cell extract, treated with EGF; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131446 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 65 kDa).
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 65 kDa). |
ICC/IF
1/100 - 1/200. |
Target
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Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
Sequence similarities
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
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Database links
- Entrez Gene: 5829 Human
- Omim: 602505 Human
- SwissProt: P49023 Human
- Unigene: 446336 Human
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Alternative names
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
Images
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All lanes : Anti-Paxillin (phospho Y88) antibody (ab131446) at 1/500 dilution
Lane 1 : HT29 cell extract, untreated
Lane 2 : HT29 cell extract, treated with EGF
Predicted band size: 65 kDa -
Immunofluorescent analysis of methanol-fixed HeLa cells labelling Paxillin (phospho Y88) with ab131446 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131446 has not yet been referenced specifically in any publications.