Key features and details
- Rabbit polyclonal to PAXX
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PAXX antibody
DescriptionRabbit polyclonal to PAXX
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC_P: Human pancreas, prostate and stomach tissue RT4, U251 MG, Human liver tissue and Human tonsil tissue lysates.
Protein target previously labeled as C9orf142.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 22 kDa.|
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
- Information by UniProt
- C9orf142 antibody
- Chromosome 9 open reading frame 142 antibody
- CI142_HUMAN antibody
All lanes : Anti-PAXX antibody (ab126353) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 22 kDa
ab126353 at a 1/1000 dilution staining PAXX in paraffin embedded Human pancreas tissue by immunohistochemistry.
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab126353 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical analysis of human stomach tissue labeling PAXX with ab126353 at 1/2500 dilution.
Immunohistochemical analysis of human prostate tissue labeling PAXX with ab126353 at 1/2500 dilution.
Immunohistochemical analysis of human skeletal muscle tissue labeling PAXX with ab126353 at 1/2500 dilution. No positivity seen in myocytes as expected.
ab126353 has been referenced in 10 publications.
- Gago-Fuentes R et al. Normal development of mice lacking PAXX, the paralogue of XRCC4 and XLF. FEBS Open Bio 8:426-434 (2018). PubMed: 29511619
- Bhargava R et al. C-NHEJ without indels is robust and requires synergistic function of distinct XLF domains. Nat Commun 9:2484 (2018). PubMed: 29950655
- Hung PJ et al. MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining. Mol Cell 71:332-342.e8 (2018). PubMed: 30017584
- Craxton A et al. PAXX and its paralogs synergistically direct DNA polymerase ? activity in DNA repair. Nat Commun 9:3877 (2018). PubMed: 30250067
- Abramowski V et al. PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice. Cell Death Differ 25:444-452 (2018). PubMed: 29077092
- Hung PJ et al. Deficiency of XLF and PAXX prevents DNA double-strand break repair by non-homologous end joining in lymphocytes. Cell Cycle 16:286-295 (2017). PubMed: 27830975
- Liu X et al. PAXX promotes KU accumulation at DNA breaks and is essential for end-joining in XLF-deficient mice. Nat Commun 8:13816 (2017). PubMed: 28051062
- Balmus G et al. Synthetic lethality between PAXX and XLF in mammalian development. Genes Dev 30:2152-2157 (2016). WB ; Mouse . PubMed: 27798842
- Lescale C et al. Specific Roles of XRCC4 Paralogs PAXX and XLF during V(D)J Recombination. Cell Rep 16:2967-2979 (2016). WB . PubMed: 27601299
- Ochi T et al. DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science 347:185-8 (2015). WB ; Human . PubMed: 25574025