Product nameAnti-PAXX antibody
DescriptionRabbit polyclonal to PAXX
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC_P: Human pancreas, prostate and stomach tissue RT4, U251 MG, Human liver tissue and Human tonsil tissue lysates.
Protein target previously labeled as C9orf142.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500. Predicted molecular weight: 22 kDa.|
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
- Information by UniProt
- C9orf142 antibody
- Chromosome 9 open reading frame 142 antibody
- CI142_HUMAN antibody
All lanes : Anti-PAXX antibody (ab126353) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 22 kDa
ab126353 at a 1/1000 dilution staining PAXX in paraffin embedded Human pancreas tissue by immunohistochemistry.
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab126353 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical analysis of human stomach tissue labeling PAXX with ab126353 at 1/2500 dilution.
Immunohistochemical analysis of human prostate tissue labeling PAXX with ab126353 at 1/2500 dilution.
Immunohistochemical analysis of human skeletal muscle tissue labeling PAXX with ab126353 at 1/2500 dilution. No positivity seen in myocytes as expected.
This product has been referenced in:
- Gago-Fuentes R et al. Normal development of mice lacking PAXX, the paralogue of XRCC4 and XLF. FEBS Open Bio 8:426-434 (2018). Read more (PubMed: 29511619) »
- Bhargava R et al. C-NHEJ without indels is robust and requires synergistic function of distinct XLF domains. Nat Commun 9:2484 (2018). Read more (PubMed: 29950655) »