Overview

  • Product name

    PBFI AM, fluorescent K+ binding probe
  • Description

    Cell-permeable, fluorescent K+ binding probe
  • Biological description

    Cell-permeable, fluorescent K+ binding probe (Kd = 5 mM). Active in vitro. Non-specific esterases hydrolyze the AM esters to produce the water-soluble form of PBFI.Excitation is 340/390 nm.

  • Purity

    > 95%
  • CAS Number

    124549-23-1
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    340nm
  • Emission

    500nm
  • Chemical name

    bis(Acetyloxymethyl)4-[6-[16-[2-[2,4-bis(acetyloxymethoxycarbonyl)phenyl]-5-methoxy-1-benzofuran-6-yl]-1,4,10,13-tetraoxa-7,16-diazacyclooctadec-7-yl]-5-methoxy-1-benzofuran-2-yl]benzene-1,3-dicarboxylate
  • Molecular weight

    1171.00
  • Molecular formula

    C58H62N2O24
  • PubChem identifier

    3283499
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Solvents listed may be unsuitable for use in biological experiments. These solvents are intended to enable solubilisation and mixing of components. We recommend that biologically unsuitable solvents are removed prior to solubilisation in experimental media.

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    For more information on AM esters please visit our AM esters FAQ page.

    Refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC(=O)OCOC(=O)C1=CC(=C(C=C1)C2=CC3=CC(=C(C=C3O2)N4CCOCCOCCN(CCOCCOCC4)C5=C(C=C6C=C(OC6=C5)C7=C(C=C(C=C7)C(=O)OCOC(=O)C)C(=O)OCOC(=O)C)OC)OC)C(=O)OCOC(=O)C
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Andersson B  et al. Induction of apoptosis by intracellular potassium ion depletion: using the fluorescent dye PBFI in a 96-well plate method in cultured lung cancer cells. Toxicol In Vitro 20:986-94 (2006). Read more (PubMed: 16483738) »
  • Laskay G  et al. Role of interleukin-3 in the regulation of intracellular K+ homeostasis in cultured murine haemopoietic cells. Biochem Biophys Res Commun 214:348-53 (1995). Read more (PubMed: 7677740) »
  • Jezek P  et al. Reconstitution of the beef heart and rat liver mitochondrial K+/H+ (Na+/H+) antiporter. Quantitation of K+ transport with the novel fluorescent probe, PBFI. J Biol Chem 265:10522-6 (1990). Read more (PubMed: 2162352) »

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