Product nameAnti-PBK/SPK antibody
See all PBK/SPK primary antibodies
DescriptionRabbit polyclonal to PBK/SPK
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Pig, Chimpanzee
Recombinant fragment within Human PBK/SPK (internal sequence). The exact sequence is proprietary.
Database link: Q96KB5
- WB: A431 and HeLa whole cell extracts. IP: A431 whole cell extract.
This product was previously labelled as PBK
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 36 kDa.|
FunctionPhosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Tissue specificityExpressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated; in a cell-cycle dependent manner at mitosis.
- Information by UniProt
- Cancer/testis antigen 84 antibody
- CT84 antibody
- Epididymis luminal protein 164 antibody
All lanes : Anti-PBK/SPK antibody (ab227239) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS-PAGE gel.
PBK/SPK was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab227239. Western blot was performed from the immunoprecipitate using ab227239. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: A431 whole cell extract.
Lane 1: Control IgG IP in A431 whole cell extract.
Lane 2: ab227239 IP in A431 whole cell extract.
ab227239 has not yet been referenced specifically in any publications.