Product nameAnti-PBK/SPK antibody
See all PBK/SPK primary antibodies
DescriptionRabbit polyclonal to PBK/SPK
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human PBK/SPK aa 200-250. The exact sequence is proprietary. NP_060962.2
Database link: Q96KB5
- WB: HeLa and HEK-293T whole cell lysate. IHC-P: Human prostate carcinoma tissue.
This product was previously labelled as PBK
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245478 was affinity purified using an epitope specific to PBK/SPK immobilized on solid support.
Our Abpromise guarantee covers the use of ab245478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPhosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Tissue specificityExpressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated; in a cell-cycle dependent manner at mitosis.
- Information by UniProt
- Cancer/testis antigen 84 antibody
- CT84 antibody
- Epididymis luminal protein 164 antibody
Formalin-fixed paraffin-embedded human prostate carcinoma tissue stained for PBK/SPK using ab245478 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
All lanes : Anti-PBK/SPK antibody (ab245478) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes
ab245478 has not yet been referenced specifically in any publications.