Overview

  • Product name

    Anti-PBK/SPK antibody - C-terminal
    See all PBK/SPK primary antibodies
  • Description

    Rabbit polyclonal to PBK/SPK - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PBK/SPK aa 306-322 (C terminal).
    Sequence:

    KDRPSAAHIVEALETDV


    Database link: Q96KB5

  • Positive control

    • Rat testis tissue lysate; HeLa, MCF7, SKOV, HepG2 and COLO320 whole cell lysates.
  • General notes

     This product was previously labelled as PBK

     

Properties

Applications

Our Abpromise guarantee covers the use of ab191146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 36 kDa.

Target

  • Function

    Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
  • Tissue specificity

    Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated; in a cell-cycle dependent manner at mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 84 antibody
    • CT84 antibody
    • Epididymis luminal protein 164 antibody
    • FLJ14385 antibody
    • HEL164 antibody
    • Lymphokine activated killer T cell originated protein kinase antibody
    • Lymphokine-activated killer T-cell-originated protein kinase antibody
    • MAPKK like protein kinase antibody
    • MAPKK-like protein kinase antibody
    • Nori 3 antibody
    • Nori-3 antibody
    • Nori3 antibody
    • PBK antibody
    • PDZ binding kinase antibody
    • PDZ-binding kinase antibody
    • Serine/threonine protein kinase antibody
    • Spermatogenesis related protein kinase antibody
    • Spermatogenesis-related protein kinase antibody
    • SPK antibody
    • T LAK cell originated protein kinase antibody
    • T-LAK cell-originated protein kinase antibody
    • TOPK antibody
    • TOPK_HUMAN antibody
    see all

Images

  • All lanes : Anti-PBK/SPK antibody - C-terminal (ab191146) at 0.5 µg/ml

    Lane 1 : Rat testis tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : SKOV cell lysate
    Lane 5 : HepG2 cell lysate
    Lane 6 : COLO320 cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 36 kDa

References

ab191146 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191146.
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