Recombinant Anti-PBK/SPK antibody [EP2520Y] (ab75987)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-PBK/SPK antibody [EP2520Y]
See all PBK/SPK primary antibodies -
Description
Rabbit monoclonal [EP2520Y] to PBK/SPK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PBK/SPK (C terminal). The exact sequence is proprietary.
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Positive control
- Human tonsil. HeLa cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as PBK
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2520Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab75987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100000 - 1/500000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). | |
| IP | 1/30. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. -
Tissue specificity
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated; in a cell-cycle dependent manner at mitosis. - Information by UniProt
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Database links
- Entrez Gene: 55872 Human
- Omim: 611210 Human
- SwissProt: Q96KB5 Human
- Unigene: 104741 Human
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Alternative names
- Cancer/testis antigen 84 antibody
- CT84 antibody
- Epididymis luminal protein 164 antibody
see all
Images
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![Western blot - Anti-PBK/SPK antibody [EP2520Y] (ab75987)](https://www.abcam.com/ps/products/75/ab75987/Images/ab75987-259084-anti-pbk-antibody-ep2520y-western-blot.jpg)
Western blot - Anti-PBK/SPK antibody [EP2520Y] (ab75987)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PBK knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human tonsil tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab75987 observed at 40 kDa. Red - loading control, ab7291, observed at 52 kDa.
ab75987 was shown to recognize PBK when PBK knockout samples were used, along with additional cross-reactive bands. Wild-type and PBK knockout samples were subjected to SDS-PAGE. ab75987 diluted to 1/100,000 and ab7291 (loading control to alpha Tubulin) diluted to 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-PBK/SPK antibody [EP2520Y] (ab75987)](https://www.abcam.com/ps/products/75/ab75987/Images/ab75987-91236-anti-pbk-antibody-ep2520y-western-blot.gif)
Western blot - Anti-PBK/SPK antibody [EP2520Y] (ab75987)Anti-PBK/SPK antibody [EP2520Y] (ab75987) at 1/500000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EP2520Y] (ab75987)](https://www.abcam.com/ps/products/75/ab75987/Images/ab75987-91235-anti-pbk-antibody-ep2520y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EP2520Y] (ab75987)Immunohistochemical analysis of paraffin-embedded human tonsil using ab75987 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Ma H et al. PBK, targeted by EVI1, promotes metastasis and confers cisplatin resistance through inducing autophagy in high-grade serous ovarian carcinoma. Cell Death Dis 10:166 (2019). Read more (PubMed: 30778048) »
- Dou X et al. PBK/TOPK mediates geranylgeranylation signaling for breast cancer cell proliferation. Cancer Cell Int 15:27 (2015). Human . Read more (PubMed: 25745361) »
