Recombinant Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)


  • Product name

    Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)]
    See all PBK/SPK primary antibodies
  • Description

    Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9)
  • Host species

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human PBK/SPK aa 1-100 (phospho T9). The exact sequence is proprietary.
    Database link: Q96KB5

  • Positive control

    • Hela lysate treated with Nocodazole; Human testis and Rat spleen tissues; Hela cells treated with Nocodazole.
  • General notes



     This product was previously labelled as PBK


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab184953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).


  • Function

    Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
  • Tissue specificity

    Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Phosphorylated; in a cell-cycle dependent manner at mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 84 antibody
    • CT84 antibody
    • Epididymis luminal protein 164 antibody
    • FLJ14385 antibody
    • HEL164 antibody
    • Lymphokine activated killer T cell originated protein kinase antibody
    • Lymphokine-activated killer T-cell-originated protein kinase antibody
    • MAPKK like protein kinase antibody
    • MAPKK-like protein kinase antibody
    • Nori 3 antibody
    • Nori-3 antibody
    • Nori3 antibody
    • PBK antibody
    • PDZ binding kinase antibody
    • PDZ-binding kinase antibody
    • Serine/threonine protein kinase antibody
    • Spermatogenesis related protein kinase antibody
    • Spermatogenesis-related protein kinase antibody
    • SPK antibody
    • T LAK cell originated protein kinase antibody
    • T-LAK cell-originated protein kinase antibody
    • TOPK antibody
    • TOPK_HUMAN antibody
    see all


  • All lanes : Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilution

    Lane 1 : Hela lysate treated with Nocodazole
    Lane 2 : Hela lysate untreated

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

  • Flow cytometric analysis of 2% paraformaldehyde-fixed, permeabilized HeLa cells treated with Nocodazole labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution (red) compared to untreated HeLa cells (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. Nocodazole-treated HeLa cells labeled using ab184953 preincubated with phospho PBK/SPK (phospho T9) peptide (blue) or non phosphopeptide (orange).


This product has been referenced in:

  • Pirovano G  et al. TOPK modulates tumour-specific radiosensitivity and correlates with recurrence after prostate radiotherapy. Br J Cancer 117:503-512 (2017). WB ; Human . Read more (PubMed: 28677687) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184953.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact

Sign up