Recombinant
RabMAb

Recombinant Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

Overview

  • Product name

    Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)]
    See all PBK/SPK primary antibodies
  • Description

    Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human PBK/SPK aa 1-100 (phospho T9). The exact sequence is proprietary.
    Database link: Q96KB5

  • Positive control

    • Hela lysate treated with Nocodazole; Human testis and Rat spleen tissues; Hela cells treated with Nocodazole.
  • General notes

     

     

     This product was previously labelled as PBK

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).

Target

  • Function

    Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
  • Tissue specificity

    Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated; in a cell-cycle dependent manner at mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 84 antibody
    • CT84 antibody
    • Epididymis luminal protein 164 antibody
    • FLJ14385 antibody
    • HEL164 antibody
    • Lymphokine activated killer T cell originated protein kinase antibody
    • Lymphokine-activated killer T-cell-originated protein kinase antibody
    • MAPKK like protein kinase antibody
    • MAPKK-like protein kinase antibody
    • Nori 3 antibody
    • Nori-3 antibody
    • Nori3 antibody
    • PBK antibody
    • PDZ binding kinase antibody
    • PDZ-binding kinase antibody
    • Serine/threonine protein kinase antibody
    • Spermatogenesis related protein kinase antibody
    • Spermatogenesis-related protein kinase antibody
    • SPK antibody
    • T LAK cell originated protein kinase antibody
    • T-LAK cell-originated protein kinase antibody
    • TOPK antibody
    • TOPK_HUMAN antibody
    see all

Images

  • All lanes : Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilution

    Lane 1 : Hela lysate treated with Nocodazole
    Lane 2 : Hela lysate untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

  • Flow cytometric analysis of 2% paraformaldehyde-fixed, permeabilized HeLa cells treated with Nocodazole labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution (red) compared to untreated HeLa cells (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. Nocodazole-treated HeLa cells labeled using ab184953 preincubated with phospho PBK/SPK (phospho T9) peptide (blue) or non phosphopeptide (orange).

References

This product has been referenced in:

  • Pirovano G  et al. TOPK modulates tumour-specific radiosensitivity and correlates with recurrence after prostate radiotherapy. Br J Cancer 117:503-512 (2017). WB ; Human . Read more (PubMed: 28677687) »
See 1 Publication for this product

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