Overview

  • Product name
  • Description
    Rabbit polyclonal to PBR
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PBR.

  • Positive control
    • This antibody gave a positive signal in Mouse Brown Adipose tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab108489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 19 kDa).

Target

  • Function
    Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.
  • Tissue specificity
    Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
  • Sequence similarities
    Belongs to the TspO/BZRP family.
  • Cellular localization
    Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Benzodiazapine receptor (peripheral) antibody
    • Benzodiazepine peripheral binding site antibody
    • BPBS antibody
    • BZRP antibody
    • DBI antibody
    • IBP antibody
    • Isoquinoline carboxamide-binding protein antibody
    • MBR antibody
    • mDRC antibody
    • Mitochondrial benzodiazepine receptor antibody
    • PBR antibody
    • PBS antibody
    • Peripheral benzodiazepine receptor antibody
    • Peripheral benzodiazepine receptor-related protein antibody
    • Peripheral type benzodiazepine receptor antibody
    • Peripheral-type benzodiazepine receptor antibody
    • pk18 antibody
    • PKBS antibody
    • PTBR antibody
    • Ptbzr antibody
    • PTBZR02 antibody
    • RATPTBZR02 antibody
    • translocator protein (18kDa) antibody
    • Translocator protein antibody
    • Tspo antibody
    • Tspo1 antibody
    • TSPOA_HUMAN antibody
    see all

Images

  • Anti-PBR antibody (ab108489) at 1 µg/ml + P0 Mouse Brown Adipose Tissue Mouse Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa
    Observed band size: 16 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    The predicted molecular weight of PBR is 19 kDa (SwissProt), however we expect to observe a banding pattern around 16 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

ab108489 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes cell line)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12% polyacrylamide gel)
Loading amount
20 µg
Specification
Cardiomyocytes cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 23 2018

Application
Western blot
Sample
Rat Cell lysate - whole cell (H9C2, cardiomyoblast cell line)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
H9C2, cardiomyoblast cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 10 2016

Abreviews
Application
Flow Cytometry
Fixation
Formaldehyde
Permeabilization
Yes - Tween 20
Sample
Horse Cell (bone marrow derived mesenchymal stromal cells)
Specification
bone marrow derived mesenchymal stromal cells
Gating Strategy
7-AAD negative cells beside debris
Preparation
Cell harvesting/tissue preparation method: By Trypsin detached and wasched plastic adherent cells from culture
Sample buffer: PBS+BSA+NaN3+goat serum+Tween20
Username

Ms. Carmen Schröck

Verified customer

Submitted Aug 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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