Anti-PBR antibody (ab108489)
Key features and details
- Rabbit polyclonal to PBR
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-PBR antibody
See all PBR primary antibodies -
Description
Rabbit polyclonal to PBR -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Horse, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- This antibody gave a positive signal in Mouse Brown Adipose tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 19 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 19 kDa). |
Target
-
Function
Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. -
Tissue specificity
Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. -
Sequence similarities
Belongs to the TspO/BZRP family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
-
Database links
- Entrez Gene: 706 Human
- Entrez Gene: 12257 Mouse
- Entrez Gene: 24230 Rat
- Omim: 109610 Human
- SwissProt: B1AH88 Human
- SwissProt: P30536 Human
- SwissProt: P50637 Mouse
- SwissProt: P16257 Rat
see all -
Alternative names
- Benzodiazapine receptor (peripheral) antibody
- Benzodiazepine peripheral binding site antibody
- BPBS antibody
see all
Images
-
Anti-PBR antibody (ab108489) at 1 µg/ml + P0 Mouse Brown Adipose Tissue Mouse Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
The predicted molecular weight of PBR is 19 kDa (SwissProt), however we expect to observe a banding pattern around 16 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab108489 has not yet been referenced specifically in any publications.