Product nameAnti-PBR antibody
See all PBR primary antibodies
DescriptionRabbit polyclonal to PBR
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide within Rat PBR aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P16257
- This antibody gave a positive result in IHC in the following FFPE tissue: Normal Rat Testes
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab154878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionResponsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.
Tissue specificityFound in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
Sequence similaritiesBelongs to the TspO/BZRP family.
Cellular localizationMitochondrion membrane.
- Information by UniProt
- Benzodiazapine receptor (peripheral) antibody
- Benzodiazepine peripheral binding site antibody
- BPBS antibody
IHC image of TPBR staining in normal Rat testes formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154878, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Qing-Feng S et al. Matrix metalloproteinase-9 and p53 involved in chronic fluorosis induced blood-brain barrier damage and neurocyte changes. Arch Med Sci 15:457-466 (2019). Read more (PubMed: 30899299) »
- Owen DR et al. TSPO mutations in rats and a human polymorphism impair the rate of steroid synthesis. Biochem J 474:3985-3999 (2017). Read more (PubMed: 29074640) »