Overview

  • Product name

    Anti-PBR antibody - C-terminal
    See all PBR primary antibodies
  • Description

    Rabbit polyclonal to PBR - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PBR (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: P30536

  • Positive control

    • WB: HeLa cell extract; U-87 MG and A431 whole cell extracts. IP: A431 whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab226783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
WB 1/500 - 1/10000. Predicted molecular weight: 19 kDa.

Target

  • Function

    Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.
  • Tissue specificity

    Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
  • Sequence similarities

    Belongs to the TspO/BZRP family.
  • Cellular localization

    Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Benzodiazapine receptor (peripheral) antibody
    • Benzodiazepine peripheral binding site antibody
    • BPBS antibody
    • BZRP antibody
    • DBI antibody
    • IBP antibody
    • Isoquinoline carboxamide-binding protein antibody
    • MBR antibody
    • mDRC antibody
    • Mitochondrial benzodiazepine receptor antibody
    • PBR antibody
    • PBS antibody
    • Peripheral benzodiazepine receptor antibody
    • Peripheral benzodiazepine receptor-related protein antibody
    • Peripheral type benzodiazepine receptor antibody
    • Peripheral-type benzodiazepine receptor antibody
    • pk18 antibody
    • PKBS antibody
    • PTBR antibody
    • Ptbzr antibody
    • PTBZR02 antibody
    • RATPTBZR02 antibody
    • translocator protein (18kDa) antibody
    • Translocator protein antibody
    • Tspo antibody
    • Tspo1 antibody
    • TSPOA_HUMAN antibody
    see all

Images

  • All lanes : Anti-PBR antibody - C-terminal (ab226783) at 1/5000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : PBR Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa

  • All lanes : Anti-PBR antibody - C-terminal (ab226783) at 1/5000 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : PBR knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa



    15% SDS-PAGE gel.

  • PBR was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab226783. Western blot was performed from the immunoprecipitate using ab226783.

    Lane 1: Control IgG IP in A431 whole cell extract.

    Lane 2: ab226783 IP in A431 whole cell extract.

  • All lanes : Anti-PBR antibody - C-terminal (ab226783) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 19 kDa



    15% SDS-PAGE gel.

References

ab226783 has not yet been referenced specifically in any publications.

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