Product namePBR overexpression 293T lysate (whole cell)
ab94211 is a 293T cell transfected lysate in which Human PBR has been transiently over-expressed using a pCMV-PBR plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituent: 100% 1x Sample Buffer
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
BackgroundFunction: Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. Tissue specificity: Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. Similarity: Belongs to the TspO/BZRP family.
Our Abpromise guarantee covers the use of ab94211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94211 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-PBR antibody (ab54895) at 1/500 dilution
Lane 1 :
PBR overexpression 293T lysate (whole cell) (ab94211)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94211 has not yet been referenced specifically in any publications.