Overview

  • Product name

    Anti-PBX1/PRL antibody [146C1a]
    See all PBX1/PRL primary antibodies
  • Description

    Mouse monoclonal [146C1a] to PBX1/PRL
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PBX1/PRL (internal sequence).

  • General notes

    Previously labelled as PBX1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab66496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 47 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function

    Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation.
  • Tissue specificity

    Expressed in all tissues except in cells of the B and T lineage.
  • Involvement in disease

    Note=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family.
  • Sequence similarities

    Belongs to the TALE/PBX homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686B09108 antibody
    • Homeo box protein PBX1 antibody
    • Homeo box protein PRL antibody
    • Homeobox protein PBX 1 antibody
    • Homeobox protein PBX1 antibody
    • Homeobox protein PRL antibody
    • MGC126627 antibody
    • PBX 1 antibody
    • Pbx1 antibody
    • PBX1_HUMAN antibody
    • Pre B cell leukemia homeobox 1 antibody
    • Pre B cell leukemia transcription factor 1 antibody
    • Pre-B-cell leukemia transcription factor 1 antibody
    • PRL antibody
    see all

Images

  • Western blot analysis of immunized recombinant protein, using ab66496.

References

ab66496 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your email last week and for your patience. I have heard back from the testing laboratory, and unfortunately the antibody has not been specifically tested against the isoform B. However, we do have a PBX1 antibody that I would expect to react with the B isoform based on the immunogen sequence- https://www.abcam.com/PBX1-antibody-ab12001.html  Please let me know if you have any questions or if there is anything else that we can do for you.

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