Anti-PBX1/PRL antibody (ab154285)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-PBX1/PRL antibody
See all PBX1/PRL primary antibodies -
Description
Rabbit polyclonal to PBX1/PRL -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human PBX1/PRL aa 1-311.
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Positive control
- A431 whole cell lysate
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General notes
Previously labelled as PBX1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
Target
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Function
Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. -
Tissue specificity
Expressed in all tissues except in cells of the B and T lineage. -
Involvement in disease
Note=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family. -
Sequence similarities
Belongs to the TALE/PBX homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395505 Chicken
- Entrez Gene: 540688 Cow
- Entrez Gene: 5087 Human
- Entrez Gene: 18514 Mouse
- Entrez Gene: 304947 Rat
- Entrez Gene: 399184 Xenopus laevis
- Omim: 176310 Human
- SwissProt: P40424 Human
see all -
Alternative names
- DKFZp686B09108 antibody
- Homeo box protein PBX1 antibody
- Homeo box protein PRL antibody
see all
Images
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Western blot - Anti-PBX1/PRL antibody (ab154285)Anti-PBX1/PRL antibody (ab154285) at 1/1000 dilution + A431 whole cell
lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE
Protocols
References
This product has been referenced in:
- Platais C et al. Targeting HOX-PBX interactions causes death in oral potentially malignant and squamous carcinoma cells but not normal oral keratinocytes. BMC Cancer 18:723 (2018). Read more (PubMed: 29980182) »
