Product nameAnti-PBX1/PRL antibody
See all PBX1/PRL primary antibodies
DescriptionRabbit polyclonal to PBX1/PRL
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Zebrafish
Synthetic peptide corresponding to Human PBX1/PRL aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HL60; K562; MOLT4.
Previously labelled as PBX1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).|
FunctionBinds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation.
Tissue specificityExpressed in all tissues except in cells of the B and T lineage.
Involvement in diseaseNote=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family.
Sequence similaritiesBelongs to the TALE/PBX homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- DKFZp686B09108 antibody
- Homeo box protein PBX1 antibody
- Homeo box protein PRL antibody
All lanes : Anti-PBX1/PRL antibody (ab97994) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 5 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lane 8 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lane 9 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lane 10 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
ICC/IF image of ab97994 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97994, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 and Hek392 cells at 5µg/ml.
This product has been referenced in:
- Zhang LX et al. uc.38 induces breast cancer cell apoptosis via PBX1. Am J Cancer Res 7:2438-2451 (2017). Read more (PubMed: 29312798) »
- Clarke-Harris R et al. PGC1a promoter methylation in blood at 5-7 years predicts adiposity from 9 to 14 years (EarlyBird 50). Diabetes 63:2528-37 (2014). Human . Read more (PubMed: 24622795) »