Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15937] to PBX1/PRL
- Suitable for: IP, WB
- Reacts with: Human
Product nameAnti-PBX1/PRL antibody [EPR15937]
See all PBX1/PRL primary antibodies
DescriptionRabbit monoclonal [EPR15937] to PBX1/PRL
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human PBX1/PRL aa 1-150. The exact sequence is proprietary.
Database link: P40424
- Hela, Jurkat, A431 cell lysates
Previously labelled as PBX1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab192606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa).|
FunctionBinds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation.
Tissue specificityExpressed in all tissues except in cells of the B and T lineage.
Involvement in diseaseNote=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family.
Sequence similaritiesBelongs to the TALE/PBX homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- DKFZp686B09108 antibody
- Homeo box protein PBX1 antibody
- Homeo box protein PRL antibody
Immunoprecipitation of Hela cell lysates (+) or PBS control (-) labeling PBX1 using ab192606 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 as secondary.
All lanes : Anti-PBX1/PRL antibody [EPR15937] (ab192606) at 1/50000 dilution
Lane 1 : Hela cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 47 kDa
Additional bands at: 38 kDa (possible isoform)
ab192606 has not yet been referenced specifically in any publications.