Recombinant Anti-PBX1/PRL antibody [EPR15937] - BSA and Azide free (ab251141)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15937] to PBX1/PRL - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PBX1/PRL antibody [EPR15937] - BSA and Azide free
See all PBX1/PRL primary antibodies -
Description
Rabbit monoclonal [EPR15937] to PBX1/PRL - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251141 is the carrier-free version of ab192606.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR15937 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa).
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. -
Tissue specificity
Expressed in all tissues except in cells of the B and T lineage. -
Involvement in disease
Note=A chromosomal aberration involving PBX1 is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with TCF3. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family. -
Sequence similarities
Belongs to the TALE/PBX homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5087 Human
- Omim: 176310 Human
- SwissProt: P40424 Human
- Unigene: 557097 Human
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Alternative names
- DKFZp686B09108 antibody
- Homeo box protein PBX1 antibody
- Homeo box protein PRL antibody
see all
Images
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All lanes : Anti-PBX1/PRL antibody [EPR15937] (ab192606) at 1/50000 dilution
Lane 1 : Hela cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 47 kDa
Additional bands at: 38 kDa (possible isoform)This data was developed using ab192606, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251141 has not yet been referenced specifically in any publications.