Key features and details
- Rabbit polyclonal to PBX2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PBX2 antibody
See all PBX2 primary antibodies
DescriptionRabbit polyclonal to PBX2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human epididymis and liver tissue WB: Human RT-4 and U-251MG cells ICC/IF: Human U-2OS cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab252996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceTranscriptional activator that binds the sequence 5'-ATCAATCAA-3'. Activates transcription of PF4 in complex with MEIS1
- G17 antibody
- Homeobox 12 antibody
- Homeobox protein PBX2 antibody
All lanes : Anti-PBX2 antibody (ab252996) at 0.4 µg/ml
Lane 2 : Human cell line RT-4
Lane 3 : Human cell line U-251MG sp
PFA-fixed, Triton X-100 permeabilized U-2OS (Human brain glioma cell line) cells stained for PBX2 (green) using ab252996 at 4 µg/ml in ICC/IF.
Paraffin-embedded human liver tissue stained for PBX2 using ab252996 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human epididymis tissue stained for PBX2 using ab252996 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab252996 has not yet been referenced specifically in any publications.