Key features and details
- Rabbit polyclonal to Pbx3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Pbx3 antibody
See all Pbx3 primary antibodies
DescriptionRabbit polyclonal to Pbx3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Horse, Chicken, Human, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
Synthetic peptide corresponding to Mouse Pbx3 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: O35317
- This antibody gave a positive signal in the following tissue lysates: E14 Mouse Embryonic Brain; E16 Mouse Embryonic Brain; P0 Mouse Brain; P7 Mouse Brain.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.|
FunctionTranscriptional activator that binds the sequence 5'-ATCAATCAA-3'.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the TALE/PBX homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
FormThere are 4 isoforms produced by alternative splicing.
- Homeobox protein PBX3 antibody
- MGC134053 antibody
- OTTHUMP00000064213 antibody
All lanes : Anti-Pbx3 antibody (ab129478) at 1 µg (Milk blocking - 3%)
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E16 Ms Embryo Brain Tissue Lysate
Lane 3 : P0 Mouse Brain Mouse Tissue Lysate
Lane 4 : P7 Mouse Brain Tissue Lysate Lab Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
The predicted molecular weight of Pbx3 is 47 kDa (SwissProt), however we expect to observe a banding pattern at 50 kDa.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129478 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab129478 has not yet been referenced specifically in any publications.