Overview

  • Product name

  • Description

    Rabbit polyclonal to Pbx3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Chicken, Human, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Mouse Pbx3 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: O35317

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: E14 Mouse Embryonic Brain; E16 Mouse Embryonic Brain; P0 Mouse Brain; P7 Mouse Brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab129478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.

Target

Images

  • All lanes : Anti-Pbx3 antibody (ab129478) at 1 µg (Milk blocking - 3%)

    Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E16 Ms Embryo Brain Tissue Lysate
    Lane 3 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 4 : P7 Mouse Brain Tissue Lysate Lab Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    The predicted molecular weight of Pbx3 is 47 kDa (SwissProt), however we expect to observe a banding pattern at 50 kDa.

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129478 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab129478 has not yet been referenced specifically in any publications.

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