Recombinant Anti-Pbx3 antibody [EPR2833(2)] (ab109173)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2833(2)] to Pbx3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Pbx3 antibody [EPR2833(2)]
See all Pbx3 primary antibodies -
Description
Rabbit monoclonal [EPR2833(2)] to Pbx3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, A431 and HeLa lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2833(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa. |
Target
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Function
Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the TALE/PBX homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5090 Human
- Entrez Gene: 18516 Mouse
- Entrez Gene: 311876 Rat
- Omim: 176312 Human
- SwissProt: P40426 Human
- SwissProt: O35317 Mouse
- Unigene: 428027 Human
- Unigene: 239941 Mouse
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Form
There are 4 isoforms produced by alternative splicing. -
Alternative names
- Homeobox protein PBX3 antibody
- MGC134053 antibody
- OTTHUMP00000064213 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab109173 has been referenced in 10 publications.
- Fu L et al. CircNRIP1 Exerts Oncogenic Functions in Papillary Thyroid Carcinoma by Sponging miR-653-5p and Regulating PBX3 Expression. J Oncol 2022:2081501 (2022). PubMed: 35646117
- Zhao X et al. circNBPF10/miR-224 Axis Regulates PBX3 to Promote the Malignant Progression of Lung Cancer. J Oncol 2022:2832920 (2022). PubMed: 35342419
- Zhang H et al. PBX3-activated DLG1-AS1 can promote the proliferation, invasion, and migration of TNBC cells by sponging miR-16-5p. Mol Ther Oncolytics 25:201-210 (2022). PubMed: 35592389
- Lu Y et al. Circular RNA circHECTD1 prevents Diosbulbin-B-sensitivity via miR-137/PBX3 axis in gastric cancer. Cancer Cell Int 21:264 (2021). PubMed: 34001137
- Mou D et al. MG132 inhibits the expression of PBX3 through miRNAs by targeting Argonaute2 in hepatoma cells. Saudi J Biol Sci 27:2157-2163 (2020). PubMed: 32714042
- Li YS et al. MicroRNA-320a suppresses tumor progression by targeting PBX3 in gastric cancer and is downregulated by DNA methylation. World J Gastrointest Oncol 11:842-856 (2019). PubMed: 31662823
- Xu X et al. PBX3/MEK/ERK1/2/LIN28/let-7b positive feedback loop enhances mesenchymal phenotype to promote glioblastoma migration and invasion. J Exp Clin Cancer Res 37:158 (2018). PubMed: 30016974
- Guo H et al. PBX3 is essential for leukemia stem cell maintenance in MLL-rearranged leukemia. Int J Cancer 141:324-335 (2017). PubMed: 28411381
- Han H et al. PBX3 is targeted by multiple miRNAs and is essential for liver tumour-initiating cells. Nat Commun 6:8271 (2015). PubMed: 26420065
- Jiang X et al. miR-495 is a tumor-suppressor microRNA down-regulated in MLL-rearranged leukemia. Proc Natl Acad Sci U S A 109:19397-402 (2012). WB . PubMed: 23132946