Overview

  • Product name
    Anti-PBXIP1 antibody - N-terminal
    See all PBXIP1 primary antibodies
  • Description
    Rabbit polyclonal to PBXIP1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human PBXIP1 aa 1-50 (N terminal). The exact sequence is proprietary. NP_065385.2
    Sequence:

    MASCPDSDNS WVLAGSESLP VETLGPASRM DPESERALQA PHSPSKTDGK


    Database link: Q96AQ6

  • Positive control
    • HeLa whole cell lysate; Human pancreatic islet cell tumor tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab176591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Relevance
    PBXIP1 is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. It inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. PBXIP1 also tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling.
  • Cellular localization
    Cytoplasm, cytoskeleton. Nucleus. Note=Shuttles between the nucleus and the cytosol. Mainly localized in the cytoplasm, associated with microtubules. Detected in small amounts in the nucleus.
  • Database links
  • Alternative names
    • 4732463H20Rik antibody
    • Hematopoietic PBX interacting protein antibody
    • HPIP antibody
    • MGC38361 antibody
    • Pre B cell leukemia homeobox interacting protein 1 antibody
    • RP11-307C12.2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreatic islet cell tumor tissue labeling PBXIP1 with ab176591 at 1µg/ml.

  • All lanes : Anti-PBXIP1 antibody - N-terminal (ab176591) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 81 kDa


    Exposure time: 10 seconds
  • Detection of PBXIP1 by Western Blot of Immunprecipitate.
    ab176591 at 0.1µg/ml labeling PBXIP1 in HeLa whole cell lysate immunoprecipitated using ab176591 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

     

References

ab176591 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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