Product nameAnti-PBXIP1 antibody - N-terminal
See all PBXIP1 primary antibodies
DescriptionRabbit polyclonal to PBXIP1 - N-terminal
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa whole cell lysate; Human pancreatic islet cell tumor tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 81 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
RelevancePBXIP1 is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. It inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. PBXIP1 also tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling.
Cellular localizationCytoplasm, cytoskeleton. Nucleus. Note=Shuttles between the nucleus and the cytosol. Mainly localized in the cytoplasm, associated with microtubules. Detected in small amounts in the nucleus.
- 4732463H20Rik antibody
- Hematopoietic PBX interacting protein antibody
- HPIP antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreatic islet cell tumor tissue labeling PBXIP1 with ab176591 at 1µg/ml.
All lanes : Anti-PBXIP1 antibody - N-terminal (ab176591) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 10 seconds
Detection of PBXIP1 by Western Blot of Immunprecipitate.
ab176591 at 0.1µg/ml labeling PBXIP1 in HeLa whole cell lysate immunoprecipitated using ab176591 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
ab176591 has not yet been referenced specifically in any publications.