Anti-PBXIP1/HPIP antibody (ab84752)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PBXIP1/HPIP antibody
See all PBXIP1/HPIP primary antibodies -
Description
Rabbit polyclonal to PBXIP1/HPIP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human PBXIP1/HPIP.
Database link: NP_079033.3 -
Positive control
- Breast carcinoma, ovarian carcinoma,anaplastic tyroid carcinoma,pancreatic islet cell tumor,stomach adenocarcinoma,testicular seminoma,stomach adenocarcinoma,prostrate carcinoma,linitis plastica stomach cancer,metastatic lymph node,teratoma
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General notes
This product was previously labelled as PBXIP1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab84752 was affinity purified using an epitope specific to HPIP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Application notes
IHC-P: 1/100 - 1/500. Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work on frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Epitope exposure with citrate buffer will enhance staining.
Likely to work on frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
PBXIP1 is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. It inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. PBXIP1 also tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling. -
Cellular localization
Cytoplasm, cytoskeleton. Nucleus. Note=Shuttles between the nucleus and the cytosol. Mainly localized in the cytoplasm, associated with microtubules. Detected in small amounts in the nucleus. -
Database links
- Entrez Gene: 57326 Human
- Entrez Gene: 229534 Mouse
- GenBank: NM_020524.2 Human
- SwissProt: Q96AQ6 Human
- SwissProt: Q3TVI8 Mouse
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Alternative names
- 4732463H20Rik antibody
- Hematopoietic PBX interacting protein antibody
- HPIP antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBXIP1/HPIP antibody (ab84752)Detection of mouse HPIP by immunohistochemistry on formalin fixed, paraffin embedded sections of mouse teratoma , using ab84752 at a 1/100 dilution.
Detection: DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBXIP1/HPIP antibody (ab84752)Detection of human HPIP by immunohistochemistry on formalin fixed, paraffin embedded sections of human ovarian carcinoma, using ab84752 at a 1/100 dilution.
Detection: DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBXIP1/HPIP antibody (ab84752)Detection of human HPIP by immunohistochemistry immunofluorescence of human ovarian carcinoma, using ab84752 at 1/100 dilution.
Detection: red fluorescent Goat anti-Rabbit IgG diluted 1/100.
Protocols
Datasheets and documents
References
ab84752 has not yet been referenced specifically in any publications.
