Key features and details
- Rabbit polyclonal to PBXIP1/HPIP - N-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PBXIP1/HPIP antibody - N-terminal
See all PBXIP1/HPIP primary antibodies
DescriptionRabbit polyclonal to PBXIP1/HPIP - N-terminal
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa whole cell lysate; Human pancreatic islet cell tumor tissue.
This product was previously labelled as PBXIP1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 81 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevancePBXIP1 is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. It inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. PBXIP1 also tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling.
Cellular localizationCytoplasm, cytoskeleton. Nucleus. Note=Shuttles between the nucleus and the cytosol. Mainly localized in the cytoplasm, associated with microtubules. Detected in small amounts in the nucleus.
- 4732463H20Rik antibody
- Hematopoietic PBX interacting protein antibody
- HPIP antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreatic islet cell tumor tissue labeling PBXIP1/HPIP with ab176591 at 1µg/ml.
All lanes : Anti-PBXIP1/HPIP antibody - N-terminal (ab176591) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 10 seconds
Detection of PBXIP1/HPIP by Western Blot of Immunprecipitate.
ab176591 at 0.1µg/ml labeling PBXIP1/HPIP in HeLa whole cell lysate immunoprecipitated using ab176591 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
ab176591 has been referenced in 1 publication.
- Cheng L et al. Overexpression of HPIP as a biomarker for metastasis and prognosis prediction in endometrial cancer patients. J Clin Lab Anal 33:e22959 (2019). PubMed: 31241209