Product namePC12 nuclear extract lysate (hypoxia treated)
See all PC12 lysates
This PC-12 extract (Hypoxia) was tested positively in Western blot and EMSA for NFKB p50 and Pax-3. Before harvesting the cells were deprived of oxygen for 4 hours. Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
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BackgroundGenerated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14886 has not yet been referenced specifically in any publications.