Anti-PC1/3 antibody (ab154246)
Key features and details
- Rabbit polyclonal to PC1/3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PC1/3 antibody
See all PC1/3 primary antibodies -
Description
Rabbit polyclonal to PC1/3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 93-390 of Human PC1/3 (Uniprot ID P29120).
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Positive control
- HepG2 and HCT116 whole cell lysates; Cal27 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab154246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/3000. Predicted molecular weight: 84 kDa. | |
IHC-P | 1/100 - 1/1000. |
Target
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Function
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. -
Involvement in disease
Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones. -
Sequence similarities
Belongs to the peptidase S8 family. Furin subfamily. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules. - Information by UniProt
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Database links
- Entrez Gene: 281967 Cow
- Entrez Gene: 5122 Human
- Entrez Gene: 18548 Mouse
- Entrez Gene: 25204 Rat
- Entrez Gene: 100005716 Zebrafish
- Omim: 162150 Human
- SwissProt: Q9GLR1 Cow
- SwissProt: P29120 Human
see all -
Alternative names
- BDP antibody
- NEC 1 antibody
- NEC1 antibody
see all
Images
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Anti-PC1/3 antibody (ab154246) at 1/500 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 84 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody (ab154246)Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labeling PC1/3 with ab154246 at 1/500.
Protocols
References (1)
ab154246 has been referenced in 1 publication.
- Morimoto K et al. Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in Male Mice. Endocrinology 157:1071-81 (2016). PubMed: 26789236