Anti-PC1/3 antibody (ab154246)

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Western blot - Anti-PC1/3 antibody (ab154246)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody (ab154246)

Key features and details

  • Rabbit polyclonal to PC1/3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PC1/3 antibody
    See all PC1/3 primary antibodies

  • Description

    Rabbit polyclonal to PC1/3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 93-390 of Human PC1/3 (Uniprot ID P29120).

  • Positive control

    • HepG2 and HCT116 whole cell lysates; Cal27 xenograft tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab154246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 84 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

  • Involvement in disease

    Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.

  • Sequence similarities

    Belongs to the peptidase S8 family. Furin subfamily.

  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BDP antibody
    • NEC 1 antibody
    • NEC1 antibody
    • NEC1_HUMAN antibody
    • Neuroendocrine convertase 1 antibody
    • PC1 antibody
    • PC3 antibody
    • PCSK1 antibody
    • Prohormone convertase 1 antibody
    • Prohormone convertase 1/3 antibody
    • Prohormone convertase 3 antibody
    • Proprotein convertase 1 antibody
    • SPC3 antibody
    see all

Images

  • Western blot - Anti-PC1/3 antibody (ab154246)
    Western blot - Anti-PC1/3 antibody (ab154246)
    Anti-PC1/3 antibody (ab154246) at 1/500 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 84 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody (ab154246)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody (ab154246)
    Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labeling PC1/3 with ab154246 at 1/500.

References (1)

Publishing research using ab154246? Please let us know so that we can cite the reference in this datasheet.

ab154246 has been referenced in 1 publication.

  • Morimoto K  et al. Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in Male Mice. Endocrinology 157:1071-81 (2016). PubMed: 26789236

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