Key features and details
- Rabbit polyclonal to PC1/3
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-PC1/3 antibody
See all PC1/3 primary antibodies
DescriptionRabbit polyclonal to PC1/3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Rat Liver Lysate, Rat Thymus Lysate, A549 Cell Lysate, HELA Cell Lysate, COLO320 Cell Lysate & PANC Cell Lysate, Rat Intestine Tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 84 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
Involvement in diseaseDefects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.
Sequence similaritiesBelongs to the peptidase S8 family. Furin subfamily.
Cellular localizationCytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
- Information by UniProt
- BDP antibody
- NEC 1 antibody
- NEC1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of PC1/3 using ab191452 in human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab191452 overnight at 4°C. DyLight®488 Conjugated Avidin was used and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat Intestine tissue labeling PC1/3 with ab191452 at 1 µg/ml.
All lanes : Anti-PC1/3 antibody (ab191452) at 0.5 µg/ml
Lane 1 : Rat Liver Tissue Lysate
Lane 2 : Rat Thymus Tissue Lysate
Lane 3 : A549 cell lysate
Lane 4 : HELA cell lysate
Lane 5 : COLO320 cell lysate
Lane 6 : PANC cell lysate
Predicted band size: 84 kDa
ab191452 has not yet been referenced specifically in any publications.