Overview

  • Product name

  • Description

    Rabbit polyclonal to PC1/3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PC1/3 aa 726-740 (C terminal).
    Sequence:

    DVFYNTKPYKHRDDR


    Database link: P29120

  • Positive control

    • Rat Liver Lysate, Rat Thymus Lysate, A549 Cell Lysate, HELA Cell Lysate, COLO320 Cell Lysate & PANC Cell Lysate, Rat Intestine Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab191452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 84 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
  • Involvement in disease

    Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.
  • Sequence similarities

    Belongs to the peptidase S8 family. Furin subfamily.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • BDP antibody
    • NEC 1 antibody
    • NEC1 antibody
    • NEC1_HUMAN antibody
    • Neuroendocrine convertase 1 antibody
    • PC1 antibody
    • PC3 antibody
    • PCSK1 antibody
    • Prohormone convertase 1 antibody
    • Prohormone convertase 1/3 antibody
    • Prohormone convertase 3 antibody
    • Proprotein convertase 1 antibody
    • SPC3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of PC1/3 using ab191452 in human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab191452 overnight at 4°C. DyLight®488 Conjugated Avidin was used and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat Intestine tissue labeling PC1/3 with ab191452 at 1 µg/ml.

  • All lanes : Anti-PC1/3 antibody (ab191452) at 0.5 µg/ml

    Lane 1 : Rat Liver Tissue Lysate
    Lane 2 : Rat Thymus Tissue Lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HELA cell lysate
    Lane 5 : COLO320 cell lysate
    Lane 6 : PANC cell lysate

    Predicted band size: 84 kDa

References

ab191452 has not yet been referenced specifically in any publications.

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