Key features and details
- Rabbit polyclonal to PC1/3
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-PC1/3 antibody
See all PC1/3 primary antibodies
DescriptionRabbit polyclonal to PC1/3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment (His-T7-tag) corresponding to Human PC1/3 aa 500-738. (Expressed in E.coli).
AIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFK NWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIVNWKLILHGTSSQPE HMKQPRVYTSYNTVQNDRRGVEKMVDPGEEQPTQENPKENTLVSKSPSSS SVGGRRDELEEGAPSQAMLRLLQSAFSKNSPPKQSPKKSPSAKLNIPYEN FYEALEKLNKPSQLKDSEDSLYNDYVDVFYNTKPYKHRD
Database link: P29120
- IHC-P: Human kidney, breast cancer, prostate cancer, liver, prostate and liver cancer tissues. WB: Pig heart, brain, kidney and liver lysates; Recombinant human PC1/3 protein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232716 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 84 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionInvolved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
Involvement in diseaseDefects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.
Sequence similaritiesBelongs to the peptidase S8 family. Furin subfamily.
Cellular localizationCytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
- Information by UniProt
- BDP antibody
- NEC 1 antibody
- NEC1 antibody
Paraffin-embedded human kidney tissue stained for PC1/3 using ab232716 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human breast cancer tissue stained for PC1/3 using ab232716 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human prostate cancer tissue stained for PC1/3 using ab232716 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver tissue stained for PC1/3 using ab232716 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human prostate tissue stained for PC1/3 using ab232716 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver cancer tissue stained for PC1/3 using ab232716 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Anti-PC1/3 antibody (ab232716) at 1 µg/ml + Recombinant human PC1/3 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 84 kDa
All lanes : Anti-PC1/3 antibody (ab232716) at 1 µg/ml
Lane 1 : Pig heart lysate
Lane 2 : Pig brain lysate
Lane 3 : Pig kidney lysate
Lane 4 : Pig liver lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 84 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232716 has not yet been referenced specifically in any publications.