Key features and details
- Rabbit polyclonal to PC4
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PC4 antibody
See all PC4 primary antibodies
DescriptionRabbit polyclonal to PC4
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey, Orangutan
- ICC/IF: U-2 OS cells. WB: RPMI-8226 cell lysate. IHC-P: Human cerebellum, tonsil, skeletal muscle and rectum tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 14 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionGeneral coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
Sequence similaritiesBelongs to the transcriptional coactivator PC4 family.
modificationsActivity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
- Information by UniProt
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PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling PC4 using ab244248 at 4 µg/ml (green) in ICC/IF.
Anti-PC4 antibody (ab244248) at 0.4 µg/ml + RPMI-8226 (Human cell line) cell lysate
Predicted band size: 14 kDa
Formalin-fixed, paraffin-embedded human tonsil tissue stained for PC4 with ab244248 at a 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for PC4 with ab244248 at a 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human rectum tissue stained for PC4 with ab244248 at a 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for PC4 with ab244248 at a 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244248 has not yet been referenced specifically in any publications.