Product nameAnti-PC4 antibody
See all PC4 primary antibodies
DescriptionRabbit polyclonal to PC4
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Orangutan, Elephant
Synthetic peptide corresponding to a region between residues 25 and 75 of human PC4, NP_006704.2.
- Whole cell lysate from Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 18, 190 kDa (predicted molecular weight: 14 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionGeneral coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
Sequence similaritiesBelongs to the transcriptional coactivator PC4 family.
modificationsActivity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
- Information by UniProt
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All lanes : Anti-PC4 antibody (ab72132) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 190 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PC4 with ab72132 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation/ Western Blot of PC4.
Lane 1: ab72132 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72132 at 0.1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds.
IHC image of ab72132 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72132, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Brzek A et al. Positive cofactor 4 (PC4) contributes to the regulation of replication-dependent canonical histone gene expression. BMC Mol Biol 19:9 (2018). Read more (PubMed: 30053800) »
- Liang XH et al. Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs. Nucleic Acids Res 46:293-313 (2018). Read more (PubMed: 29165591) »