Product namepCambia1201 Plant Expression Vector
Vector contains hygromycin B resistance for plant selection, chloramphenicol resistance for bacterial selection and gusA reporter gene.
The pCambia1201 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with chloramphenicol
Plant selection with hygromycin B
Simple means to construct translational fusions to gusA reporter genes
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.
This vector contains a fully functional gusA reporter construct for simple and sensitive analysis of gene function or presence in regenerated plants by GUS assay. The construct uses E.coli gusA with an intron (from the castor bean catalase gene) inside the coding sequence to ensure that expression of glucuronidase activity is derived from eukaryotic cells, not from expression by residual A.tumefaciens cells. This vector is suitable for insertion of other genes of interest containing their own promoter and terminator. Researchers can excise the gusA gene and insert their own gene of interest in its place or use this vector to create fusions of gusA with their gene of interest. This vector contain the pUC18 polylinker-lacZa.
Concentration of Antibiotics for selection.
In bacterial system:
Chloramphenicol resistance (cat – chloramphenicol acetyltransferase from E.coli Tn-cam204) in bacteria: use 10μg/mL in E.coli and A.tumefaciens.
In plant system:
Resistance Gene hph (From a Klebsiella species, via E.coli) Transformed plant selection antibiotic Hygromycin B Concentration in Arabidopsis 50 μg/mL in germination medium Concentration in Rice 50 μg/mL in 2N6 then in RGH6 Concentration in Tobacco 50 μg/mL in RMOP then in MST
Storage instructionsShipped at 4°C. Store at -20°C.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab275773 has not yet been referenced specifically in any publications.