Product namepCambia2300 Plant Expression Vector
Vector contains genes for bacterial and plant selection with kanamycin and and no reporter gene to allow construction of user's own system.
The pCambia2300 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with kanamycin
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal.
This vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes.
The pUC18 polylinker within the lacZa fragment allows blue/white screening of clones in E.coli cloning work.
Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (aphIII/nptIII/3’5’’-aminoglycoside phosphotransferase typeIII from Enterococcus faecalis pJH1) in bacteria: use 50 μg/mL in E.coli and A.tumefaciens.
In plant system:
nptII/aph(3')II from Tn5
Transformed plant selection antibiotic
Concentration in Arabidopsis
20 - 50 μg/mL
Storage instructionsShipped at 4°C. Store at -20°C.
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