Anti-PCB antibody [10G7BF7] (ab123451)

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Western blot - Anti-PCB antibody [10G7BF7] (ab123451)
  • Immunocytochemistry - Anti-PCB antibody [10G7BF7] (ab123451)

Key features and details

  • Mouse monoclonal [10G7BF7] to PCB
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-PCB antibody [10G7BF7]
    See all PCB primary antibodies

  • Description

    Mouse monoclonal [10G7BF7] to PCB

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • HepG2, Rat H9C2, Rat H4IIE, Mouse MEF cell lysates; Purified Cow liver PCB; HDFn cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab123451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 130 kDa.
ICC Use a concentration of 1 µg/ml.

Target

  • Function

    Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.

  • Pathway

    Carbohydrate biosynthesis; gluconeogenesis.

  • Involvement in disease

    Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.

  • Sequence similarities

    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • mitochondrial antibody
    • OTTHUMP00000235155 antibody
    • OTTHUMP00000235156 antibody
    • PC antibody
    • PCB antibody
    • Pcx antibody
    • PYC_HUMAN antibody
    • Pyruvate carboxylase antibody
    • Pyruvate carboxylase mitochondrial antibody
    • Pyruvic carboxylase antibody
    see all

Images

  • Western blot - Anti-PCB antibody [10G7BF7] (ab123451)
    Western blot - Anti-PCB antibody [10G7BF7] (ab123451)
    All lanes : Anti-PCB antibody [10G7BF7] (ab123451) at 1 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : Hela
    Lane 3 : H9C2
    Lane 4 : MEF
    Lane 5 : Recombinant PC

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat-anti-Mouse HRP at 1/3000 dilution

    Predicted band size: 130 kDa

  • Immunocytochemistry - Anti-PCB antibody [10G7BF7] (ab123451)
    Immunocytochemistry - Anti-PCB antibody [10G7BF7] (ab123451)
    ab123451 at 1µg/ml staining PCB in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).

References (0)

Publishing research using ab123451? Please let us know so that we can cite the reference in this datasheet.

ab123451 has not yet been referenced specifically in any publications.

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