Key features and details
- Rabbit polyclonal to PCB
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PCB antibody
See all PCB primary antibodies
DescriptionRabbit polyclonal to PCB
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human PCB aa 74-371. (BC011617).
QMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSE RADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAP ITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSE ALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQK VVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFI EVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRING
Database link: P11498
- Raji, HEK293 and 293T cell lysates, Human fetal brain tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 130 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionPyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
PathwayCarbohydrate biosynthesis; gluconeogenesis.
Involvement in diseaseDefects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
Sequence similaritiesContains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- mitochondrial antibody
- OTTHUMP00000235155 antibody
- OTTHUMP00000235156 antibody
All lanes : Anti-PCB antibody (ab196277) at 1/500 dilution
Lane 1 : Raji cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : 293T cell lysate
Predicted band size: 130 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal brain tissue labeling PCB with ab196277 at 1/100 dilution.
ab196277 has not yet been referenced specifically in any publications.