Product nameAnti-PCB antibody
See all PCB primary antibodies
DescriptionRabbit polyclonal to PCB
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- WB; Liver tissue extract. ICC/IF; MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/250. Predicted molecular weight: 130 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionPyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
PathwayCarbohydrate biosynthesis; gluconeogenesis.
Involvement in diseaseDefects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
Sequence similaritiesContains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- mitochondrial antibody
- OTTHUMP00000235155 antibody
- OTTHUMP00000235156 antibody
All lanes : Anti-PCB antibody (ab221080) at 1/100 dilution
Lane 1 : RT4 cell extract
Lane 2 : U-251 MG cell extract
Lane 3 : Human plasma
Lane 4 : Human liver tissue extract
Developed using the ECL technique.
Predicted band size: 130 kDa
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling PCB with ab221080 at 4 µg/ml. Antibody staining is shown in green.
ab221080 has not yet been referenced specifically in any publications.