The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000.
1/100 - 1/1000.
1/500 - 1/3000.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Carbohydrate biosynthesis; gluconeogenesis.
Involvement in disease
Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
Paraffin-embedded mouse liver tissue stained for PCB using ab229266 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-PCB antibody - C-terminal (ab229266)
All lanes : Anti-PCB antibody - C-terminal (ab229266) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 4 : Mouse liver tissue lysate
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PCB using ab229266 (green) at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes. Blue: Hoechst 33342 staining.
has not yet been referenced specifically in any publications.
Publishing research using ab229266? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Get resources and offers direct to your inboxSign up