Overview

  • Product name
    Anti-PCB antibody - C-terminal
    See all PCB primary antibodies
  • Description
    Rabbit polyclonal to PCB - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PCB (C terminal). The exact sequence is proprietary.
    Database link: P11498

  • Positive control
    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract. IHC-P: Rat duodenum tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 130 kDa.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/857.

Target

  • Function
    Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
  • Pathway
    Carbohydrate biosynthesis; gluconeogenesis.
  • Involvement in disease
    Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
  • Sequence similarities
    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • mitochondrial antibody
    • OTTHUMP00000235155 antibody
    • OTTHUMP00000235156 antibody
    • PC antibody
    • PCB antibody
    • Pcx antibody
    • PYC_HUMAN antibody
    • Pyruvate carboxylase antibody
    • Pyruvate carboxylase mitochondrial antibody
    • Pyruvic carboxylase antibody
    see all

Images

  • PCB was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5μg ab229267. Western blot was performed from the immunoprecipitate using ab229267. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: HeLa whole cell extract.

    Lane 2: Control IgG instead of ab229267 in HeLa whole cell extract.

    Lane 3: ab229267 IP in HeLa whole cell extract.

  • All lanes : Anti-PCB antibody - C-terminal (ab229267) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 130 kDa



    5% SDS-PAGE gel.

  • 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PCB (green) using ab229267 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded rat duodenum tissue stained for PCB using ab229267 at 1/500 dilution in immunohistochemical analysis.

References

ab229267 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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