• Product name
    Anti-PCB antibody [EPR7366]
    See all PCB primary antibodies
  • Description
    Rabbit monoclonal [EPR7366] to PCB
  • Host species
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PCB aa 1050-1150. The exact sequence is proprietary.

  • Positive control
    • Human fetal kidney and liver, HepG2, and A549 lysates; Mouse and Rat brain lysates.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab126707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
    • Pathway
      Carbohydrate biosynthesis; gluconeogenesis.
    • Involvement in disease
      Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
    • Sequence similarities
      Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • mitochondrial antibody
      • OTTHUMP00000235155 antibody
      • OTTHUMP00000235156 antibody
      • PC antibody
      • PCB antibody
      • Pcx antibody
      • PYC_HUMAN antibody
      • Pyruvate carboxylase antibody
      • Pyruvate carboxylase mitochondrial antibody
      • Pyruvic carboxylase antibody
      see all


    • All lanes : Anti-PCB antibody [EPR7366] (ab126707) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate.
      Lane 2 : PCB knockout HAP1 whole cell lysate.
      Lane 3 : HepG2 whole cell lysate.
      Lane 4 : A549 whole cell lysate.

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 130 kDa

      Lanes 1 - 4: Merged signal (red and green). Green - ab126707 observed at 130 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab126707 was shown to recognize PBC in wild-type HAP1 cells as signal was lost at the expected MW in PBC knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PBC knockout samples were subjected to SDS-PAGE. Ab126707 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-PCB antibody [EPR7366] (ab126707) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : A549 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 130 kDa

    • All lanes : Anti-PCB antibody [EPR7366] (ab126707) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Predicted band size: 130 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Kitazawa S  et al. Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors. Oncotarget 8:28922-28938 (2017). WB . Read more (PubMed: 28423651) »
    • Cardaci S  et al. Pyruvate carboxylation enables growth of SDH-deficient cells by supporting aspartate biosynthesis. Nat Cell Biol 17:1317-26 (2015). WB . Read more (PubMed: 26302408) »

    See all 2 Publications for this product

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