Recombinant
RabMAb

Recombinant Anti-PCBD1 antibody [EPR9760(B)] (ab138518)

Overview

  • Product name

    Anti-PCBD1 antibody [EPR9760(B)]
    See all PCBD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9760(B)] to PCBD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PCBD1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human ovary cancer, fetal heart, fetal liver, and Caco-2 cell lysates , Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab138518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.

Target

  • Function

    Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity.
  • Involvement in disease

    Defects in PCBD1 are the cause of BH4-deficient hyperphenylalaninemia type D (HPABH4D) [MIM:264070]; also known as hyperphenylalaninemia with primapterinuria. HPABH4D is characterized by the excretion of 7-substituted pterins in the urine of affected patients.
  • Sequence similarities

    Belongs to the pterin-4-alpha-carbinolamine dehydratase family.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic and/or nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4 alpha hydroxy tetrahydropterin dehydratase antibody
    • 4-alpha-hydroxy-tetrahydropterin dehydratase antibody
    • 6 pyruvoyl tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) antibody
    • 6 pyruvoyl tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha antibody
    • DCoH antibody
    • Dimerization cofactor of hepatic nuclear factor 1 alpha antibody
    • Dimerization cofactor of hepatocyte nuclear factor 1 alpha antibody
    • Dimerization cofactor of hepatocyte nuclear factor 1-alpha antibody
    • Dimerization cofactor of HNF1 antibody
    • Dimerizing cofactor for HNF1 antibody
    • PCBD 1 antibody
    • PCBD antibody
    • PCBD1 antibody
    • PCD antibody
    • Phenylalanine hydroxylase stimulating protein antibody
    • Phenylalanine hydroxylase-stimulating protein antibody
    • PHS antibody
    • PHS_HUMAN antibody
    • Pterin 4 alpha carbinolamine dehydratase antibody
    • Pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) antibody
    • Pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha antibody
    • Pterin carbinolamine dehydratase antibody
    • Pterin-4-alpha-carbinolamine dehydratase antibody
    see all

Images

  • All lanes : Anti-PCBD1 antibody [EPR9760(B)] (ab138518) at 1/1000 dilution

    Lane 1 : Human ovary
    cancer cell lysate
    Lane 2 : Fetal heart cell lysate
    Lane 3 : Fetal liver cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling PCBD1 with ab138518 antibody at a dilution of 1/50.

References

ab138518 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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