Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9760(B)] to PCBD1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-PCBD1 antibody [EPR9760(B)]
See all PCBD1 primary antibodies
DescriptionRabbit monoclonal [EPR9760(B)] to PCBD1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PCBD1 aa 1-100 (N terminal). The exact sequence is proprietary.
- Human ovary cancer, fetal heart, fetal liver, and Caco-2 cell lysates , Human colon tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab138518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity.
Involvement in diseaseDefects in PCBD1 are the cause of BH4-deficient hyperphenylalaninemia type D (HPABH4D) [MIM:264070]; also known as hyperphenylalaninemia with primapterinuria. HPABH4D is characterized by the excretion of 7-substituted pterins in the urine of affected patients.
Sequence similaritiesBelongs to the pterin-4-alpha-carbinolamine dehydratase family.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic and/or nuclear.
- Information by UniProt
- 4 alpha hydroxy tetrahydropterin dehydratase antibody
- 4-alpha-hydroxy-tetrahydropterin dehydratase antibody
- 6 pyruvoyl tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) antibody
All lanes : Anti-PCBD1 antibody [EPR9760(B)] (ab138518) at 1/1000 dilution
Lane 1 : Human ovary
cancer cell lysate
Lane 2 : Fetal heart cell lysate
Lane 3 : Fetal liver cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling PCBD1 with ab138518 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab138518 has not yet been referenced specifically in any publications.