Overview

  • Product name

  • Description

    Goat polyclonal to PCBP1
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide: LDLAKLNQVARQQSH by a Cysteine residue linker, corresponding to internal sequence amino acids 234-247 of Human PCBP1

  • Positive control

    • HeLa and Human Placenta lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa).

Target

  • Function

    Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
  • Tissue specificity

    Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
  • Sequence similarities

    Contains 3 KH domains.
  • Post-translational
    modifications

    Phosphorylated; lowers poly(rC)-binding activity.
  • Cellular localization

    Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha-CP1 antibody
    • Heterogeneous nuclear ribonucleoprotein E1 antibody
    • heterogenous nuclear ribonucleoprotein E1 antibody
    • heterogenous nuclear ribonucleoprotein X antibody
    • hnRNP E1 antibody
    • hnRNP-E1 antibody
    • hnRNP-X antibody
    • HNRPE1 antibody
    • HNRPX antibody
    • nucleic acid binding protein sub 2.3 antibody
    • Nucleic acid- binding protein SUB2.3 antibody
    • Nucleic acid-binding protein SUB2.3 antibody
    • PCBP1 antibody
    • PCBP1_HUMAN antibody
    • poly(rC) binding protein 1 antibody
    • Poly(rC)-binding protein 1 antibody
    • rCbinding protein 1 antibody
    see all

Images

  • Immunohistochemistry of Human Lung staning PCBP1 using ab109577 at 5 μg/ml. Steamed antigen retrieval with citrate buffer ph 6.

  • Anti-PCBP1 antibody (ab109577) at 0.01 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

References

ab109577 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109577.
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