Recombinant Anti-PCBP1 antibody [EPR11049(B)] (ab168377)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11049(B)] to PCBP1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCBP1 antibody [EPR11049(B)]
See all PCBP1 primary antibodies -
Description
Rabbit monoclonal [EPR11049(B)] to PCBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human PCBP1.
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Positive control
- HeLa, HepG2, K562 and 293T cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11049(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. -
Tissue specificity
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands. -
Sequence similarities
Contains 3 KH domains. -
Post-translational
modificationsPhosphorylated; lowers poly(rC)-binding activity. -
Cellular localization
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5093 Human
- Entrez Gene: 23983 Mouse
- Entrez Gene: 500242 Rat
- Omim: 601209 Human
- SwissProt: Q15365 Human
- SwissProt: P60335 Mouse
- Unigene: 2853 Human
- Unigene: 274146 Mouse
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Alternative names
- Alpha-CP1 antibody
- Heterogeneous nuclear ribonucleoprotein E1 antibody
- heterogenous nuclear ribonucleoprotein E1 antibody
see all
Images
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All lanes : Anti-PCBP1 antibody [EPR11049(B)] (ab168377) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa -
Western blot analysis on HeLa cell lysate pellet immunoprecipitated using ab168377 at a 1/10 dilution, and an HRP-conjugated anti-rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab168377 has been referenced in 4 publications.
- Guo S et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446
- Yang J et al. The Identification of Long Non-coding RNA H19 Target and Its Function in Chronic Myeloid Leukemia. Mol Ther Nucleic Acids 19:1368-1378 (2020). PubMed: 32160707
- Lu Y et al. SNHG1 Inhibits ox-LDL-Induced Inflammatory Response and Apoptosis of HUVECs via Up-Regulating GNAI2 and PCBP1. Front Pharmacol 11:703 (2020). PubMed: 32536864
- Horiguchi H et al. Extracellular vesicle miR-7977 is involved in hematopoietic dysfunction of mesenchymal stromal cells via poly(rC) binding protein 1 reduction in myeloid neoplasms. Haematologica 101:437-47 (2016). PubMed: 26802051