Recombinant Anti-PCBP1 antibody [EPR11049(B)] - BSA and Azide free (ab249469)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11049(B)] to PCBP1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PCBP1 antibody [EPR11049(B)] - BSA and Azide free
See all PCBP1 primary antibodies -
Description
Rabbit monoclonal [EPR11049(B)] to PCBP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PCBP1. The exact sequence is proprietary.
Database link: Q15365 -
General notes
ab249469 is the carrier-free version of ab168377.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11049(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. -
Tissue specificity
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands. -
Sequence similarities
Contains 3 KH domains. -
Post-translational
modificationsPhosphorylated; lowers poly(rC)-binding activity. -
Cellular localization
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5093 Human
- Entrez Gene: 23983 Mouse
- Entrez Gene: 500242 Rat
- Omim: 601209 Human
- SwissProt: Q15365 Human
- SwissProt: P60335 Mouse
- Unigene: 2853 Human
- Unigene: 274146 Mouse
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Alternative names
- Alpha-CP1 antibody
- Heterogeneous nuclear ribonucleoprotein E1 antibody
- heterogenous nuclear ribonucleoprotein E1 antibody
see all
Images
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All lanes : Anti-PCBP1 antibody [EPR11049(B)] (ab168377) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDaThis data was developed using ab168377, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249469 has not yet been referenced specifically in any publications.