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Rabbit monoclonal [EPR11055] to PCBP1
IP more details Unsuitable for:
Flow Cyt,ICC or IHC-P
Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human PCBP1 (UniProt: Q15365).
HeLa, 293T, HepG2 and K562 cell lysates.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 37 kDa.
1/10 - 1/100.
Is unsuitable for Flow Cyt,ICC or IHC-P.
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
Contains 3 KH domains.
Phosphorylated; lowers poly(rC)-binding activity.
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
Information by UniProt
Heterogeneous nuclear ribonucleoprotein E1 antibody
heterogenous nuclear ribonucleoprotein E1 antibody
Western blot - Anti-PCBP1 antibody [EPR11055] (ab168378)
All lanes : Anti-PCBP1 antibody [EPR11055] (ab168378) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lane 3 : HepG2 cell lysate Lane 4 : K562 cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 37 kDa
Immunoprecipitation - Anti-PCBP1 antibody [EPR11055] (ab168378)
Detection of PCBP1 by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168378 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"