Key features and details
- Mouse monoclonal [376CT13.1.2] to PCBP2/hnRNP E2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-PCBP2/hnRNP E2 antibody [376CT13.1.2]
See all PCBP2/hnRNP E2 primary antibodies
DescriptionMouse monoclonal [376CT13.1.2] to PCBP2/hnRNP E2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human PCBP2/hnRNP E2 aa 1 to the C-terminus.
- This antibody gave a positive signal in the following whole cell lysates: K562; HeLa; HepG2; Raji.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab61334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Abcam reccomends blocking with 3% milk.|
FunctionSingle-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.
Tissue specificityDetected in all tissues examined.
Sequence similaritiesContains 3 KH domains.
modificationsPhosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
Cellular localizationNucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
- Information by UniProt
- Alpha CP2 antibody
- Alpha-CP2 antibody
- alphaCP-2 antibody
All lanes : Anti-PCBP2/hnRNP E2 antibody [376CT13.1.2] (ab61334) at 5 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 110 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Abcam recommends using milk as the blocking agent.
ab61334 has not yet been referenced specifically in any publications.