Key features and details
- Rabbit polyclonal to PCBP2/hnRNP E2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PCBP2/hnRNP E2 antibody
See all PCBP2/hnRNP E2 primary antibodies
DescriptionRabbit polyclonal to PCBP2/hnRNP E2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to a region within amino acids 85-365 of Human PCBP2/hnRNP E2 (Q15366).
- Jurkat, Raji, K562 and NCI-H929 whole cell lysates. HeLa cells. Human muscle tissue.
General notesKeep as concentrated solution.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0|
|ICC/IF||1/100 - 1/1000.|
FunctionSingle-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.
Tissue specificityDetected in all tissues examined.
Sequence similaritiesContains 3 KH domains.
modificationsPhosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
Cellular localizationNucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
- Information by UniProt
- Alpha CP2 antibody
- Alpha-CP2 antibody
- alphaCP-2 antibody
All lanes : Anti-PCBP2/hnRNP E2 antibody (ab154245) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : NCI H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling PCBP2/hnRNP E2 with ab154245 at 1/500. Lower panel shows costaining with Hoechst 33342.
Immunohistochemical analysis of paraffin embedded HeLa xenograft tissue labeling PCBP2/hnRNP E2 with ab154245 at 1/500.
Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling PCBP2/hnRNP E2 with ab154245 at 1/500.
ab154245 has not yet been referenced specifically in any publications.