Anti-PCBP2/hnRNP E2 antibody (ab96169)

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Western blot - Anti-PCBP2/hnRNP E2 antibody (ab96169)

    Key features and details

    • Rabbit polyclonal to PCBP2/hnRNP E2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-PCBP2/hnRNP E2 antibody
      See all PCBP2/hnRNP E2 primary antibodies

    • Description

      Rabbit polyclonal to PCBP2/hnRNP E2

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow, Zebrafish

    • Immunogen

      Recombinant fragment, corresponding to a region within N-terminal amino acids 1-163 of Human PCBP2/hnRNP E2.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab96169 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.

    Target

    • Function

      Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.

    • Tissue specificity

      Detected in all tissues examined.

    • Sequence similarities

      Contains 3 KH domains.

    • Post-translational
      modifications

      Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.

    • Cellular localization

      Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Alpha CP2 antibody
      • Alpha-CP2 antibody
      • alphaCP-2 antibody
      • Cbp antibody
      • CTBP antibody
      • Heterogeneous nuclear ribonucleoprotein E2 antibody
      • Heterogenous nuclear ribonucleoprotein E2 antibody
      • hnRNP E2 antibody
      • hnRNP-E2 antibody
      • HNRNPE2 antibody
      • Hnrnpx antibody
      • HNRPE2 antibody
      • Hnrpx antibody
      • MGC110998 antibody
      • PCBP2 antibody
      • PCBP2_HUMAN antibody
      • poly(rC) binding protein 2 antibody
      • Poly(rC)-binding protein 2 antibody
      • Putative heterogeneous nuclear ribonucleoprotein X antibody
      • rCbinding protein 2 antibody
      see all

    Images

    • Western blot - Anti-PCBP2/hnRNP E2 antibody (ab96169)
      Western blot - Anti-PCBP2/hnRNP E2 antibody (ab96169)
      Anti-PCBP2/hnRNP E2 antibody (ab96169) at 1/1000 dilution + Raji whole cell lysate at 30 µg

      Predicted band size: 39 kDa



      10% SDS PAGE

    References (0)

    Publishing research using ab96169? Please let us know so that we can cite the reference in this datasheet.

    ab96169 has not yet been referenced specifically in any publications.

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    Western blot abreview for Anti-PCBP2/hnRNP E2 antibody

     Inconclusive
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa, 293T)
    Loading amount
    25 µg
    Specification
    HeLa, 293T
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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    Abcam user community

    Verified customer

    Submitted Apr 25 2012

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