Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, 293T)
Loading amount
25 µg
Specification
HeLa, 293T
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 30 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: cell signaling
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL, ECL+
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Additional data
Additional Notes
This antibody was rated inconclusive because the bands observed were not at the expected size. However, no siRNA treatment was tested. Thus, it is not possible to conclude on the antibody specificity.
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Submitted Apr 25 2012