Recombinant
RabMAb

Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)

Overview

  • Product name
    Anti-PCBP2/hnRNP E2 antibody [EPR14858]
    See all PCBP2/hnRNP E2 primary antibodies
  • Description
    Rabbit monoclonal [EPR14858] to PCBP2/hnRNP E2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IP, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PCBP2/hnRNP E2 aa 150-250. The exact sequence is proprietary.
    Database link: Q15366

  • Positive control
    • HeLa, Raji and K562 cell lysates; Human clear cell carcinoma of kidney tissue; Human skin tissue; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/110.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/40 - 1/60.
ICC/IF 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 35, 39 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.
  • Tissue specificity
    Detected in all tissues examined.
  • Sequence similarities
    Contains 3 KH domains.
  • Post-translational
    modifications
    Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
  • Cellular localization
    Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha CP2 antibody
    • Alpha-CP2 antibody
    • alphaCP-2 antibody
    • Cbp antibody
    • CTBP antibody
    • Heterogeneous nuclear ribonucleoprotein E2 antibody
    • Heterogenous nuclear ribonucleoprotein E2 antibody
    • hnRNP E2 antibody
    • hnRNP-E2 antibody
    • HNRNPE2 antibody
    • Hnrnpx antibody
    • HNRPE2 antibody
    • Hnrpx antibody
    • MGC110998 antibody
    • PCBP2 antibody
    • PCBP2_HUMAN antibody
    • poly(rC) binding protein 2 antibody
    • Poly(rC)-binding protein 2 antibody
    • Putative heterogeneous nuclear ribonucleoprotein X antibody
    • rCbinding protein 2 antibody
    see all

Images

  • All lanes : Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : K562 cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemical analysis of paraffin-embedded human skin tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/250 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow cytometric anlaysis of HeLa cells (2% paraformaldehyde-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunoprecipitation analysis of HeLa lysates labeling PCBP2/hnRNP E2 with ab18492 at 1/50 dilution (Lane 1) and PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used as secondary antibody. 

References

ab184962 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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